Glycosidase susceptibility: a probe for the distribution of glycoprotein oligosaccharides in Sindbis virus.
AUTOR(ES)
McCarthy, M
RESUMO
Intact Sindbis virus and Triton-solubilized viral glycoprotein were treated with alpha-mannosidase and with a preparation of mixed glycosidases from Diplococcus pneumoniae to probe the accesibility of carbohydrate units on the viral surface. The products of glycosidase attack on Triton-solubilized virus showed that mose carbohydrate units of the glycoproteins are good substrates for these enzymes. The relative resistance of most of the viral oligosaccharides in intact virus particles showed that much of the carbohydrate is not accessible to glycosidases, probably because it is not exposed at the viral surface. The only completely accessible carbohydrate units on Sindbis glycoproteins were the type A oligosaccharides of E2. This differential accessibility of Sindbis oligosaccharides is discussed in relation to the organization of the viral surface.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=515800Documentos Relacionados
- Oligosaccharides of the glycoprotein of rabies virus.
- Mutational analysis of a virulence locus in the E2 glycoprotein gene of Sindbis virus.
- Requirement for host transcription in the replication of Sindbis virus.
- Glycoproteins of Sindbis Virus: Preliminary Characterization of the Oligosaccharides
- Role of N-linked oligosaccharides in processing and intracellular transport of E2 glycoprotein of rubella virus.