Glycosyltransferases in human blood: I. Galactosyltransferase in human serum and erythrocyte membranes
AUTOR(ES)
Kim, Young S.
RESUMO
Human serum and hemoglobin-free erythrocyte membranes were found to contain a galactosyltransferase which catalyzes the transfer of galactose from UDP-galactose to specific large and small molecular weight acceptors. The requirements for enzyme activity were found to be similar for the enzymes from both sources. However, the membrane-bound enzyme depended on a detergent for maximal activity. Mn++ was an absolute requirement for transfer and uridine nucleoside phosphates were inhibitors. The most effective acceptor for galactose was a glycoprotein containing N-acetylglucosamine residues in the terminal position of its oligosaccharide side chains, N-acetylglucosamine was also an acceptor. While the presence of α-lactalbumin in the incubation medium resulted in a significant decrease in the transfer of galactose to N-acetylglucosamine, glucose, which was not an acceptor for galactose in the absence of α-lactalbumin, became an excellent acceptor. The serum enzyme catalyzed the transfer of 54 nmoles of galactose per milliliter of serum per hour and its apparent Km for UDP-galactose was 7.5 × 10-6M. The membrane enzyme had a similar apparent Km. Using a quantitative assay system the enzyme was found to be present in all individuals studied, regardless of their blood type, secretor status, or sex.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=292358Documentos Relacionados
- Glycosyltransferases in human blood: II. Study of serum galactosyltransferase and N-acetylgalactosaminyltransferase in patients with liver diseases
- Studies on Penicillin in Blood: I. The Effects of Serum and Serum Filtrates upon the Growth of Staphylococcus aureus
- N-Acetyl-D-Galactosaminyltransferase in Human Serum and Erythrocyte Membranes
- Transport of Monosaccharides I. ASYMMETRY IN THE HUMAN ERYTHROCYTE MECHANISM
- I. THE SEPARATION OF SODIUM FROM POTASSIUM IN HUMAN BLOOD SERUM BY ION EXCHANGE CHROMATOGRAPHY