Golgi-dependent transport of cholesterol to the Chlamydia trachomatis inclusion
AUTOR(ES)
Carabeo, Reynaldo A.
FONTE
National Academy of Sciences
RESUMO
Cholesterol, a lipid not normally found in prokaryotes, was identified in purified Chlamydia trachomatis elementary bodies and in the chlamydial parasitophorous vacuole (inclusion) membrane of infected HeLa cells. Chlamydiae obtained eukaryotic host cell cholesterol both from de novo synthesis or low-density lipoprotein. Acquisition of either de novo-synthesized cholesterol or low-density lipoprotein-derived cholesterol was microtubule-dependent and brefeldin A-sensitive, indicating a requirement for the Golgi apparatus. Transport also required chlamydial protein synthesis, indicative of a pathogen-directed process. The cholesterol trafficking pathway appears to coincide with a previously characterized delivery of sphingomyelin to the inclusion in that similar pharmacological treatments inhibited transport of both sphingomyelin and cholesterol. These results support the hypothesis that sphingomyelin and cholesterol may be cotransported via a Golgi-dependent pathway and that the chlamydial inclusion receives cholesterol preferentially from a brefeldin A-sensitive pathway of cholesterol trafficking from the Golgi apparatus to the plasma membrane.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=164522Documentos Relacionados
- Lipid metabolism in Chlamydia trachomatis-infected cells: directed trafficking of Golgi-derived sphingolipids to the chlamydial inclusion.
- Endosome to Golgi Transport of Ricin Is Regulated by Cholesterol
- The atherogenic effects of chlamydia are dependent on serum cholesterol and specific to Chlamydia pneumoniae
- Isolates of Chlamydia trachomatis That Occupy Nonfusogenic Inclusions Lack IncA, a Protein Localized to the Inclusion Membrane
- Dissecting the role of the Golgi complex and lipid rafts in biosynthetic transport of cholesterol to the cell surface