Grafting fibroblasts genetically modified to produce L-dopa in a rat model of Parkinson disease.
AUTOR(ES)
Wolff, J A
RESUMO
Rat fibroblasts were infected with a retroviral vector containing the cDNA for rat tyrosine hydroxylase [TH; tyrosine 3-monooxygenase; L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2]. A TH-positive clone was identified by biochemical assay and immunohistochemical staining. When supplemented in vitro with pterin cofactors required for TH activity, these cells produced L-dopa and released it into the cell culture medium. Uninfected control cells and fibroblasts infected with the TH vector were grafted separately to the caudate of rats with unilateral 6-hydroxydopamine lesions of the nigrostriatal pathway. Only grafts containing TH-expressing fibroblasts were found to reduce rotational asymmetry. These results have general implications for the application of gene therapy to human neurological disease and specific implications for Parkinson disease.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=298422Documentos Relacionados
- Bromocriptine alone or associated with L-dopa plus benserazide in Parkinson's disease.
- Influence of dystonia on the response to long-term L-dopa therapy in Parkinson's disease
- An analysis of six patients with Parkinson's disease who have been unresponsive to L-dopa therapy
- L-dopa and arousal 1
- Quantitative study of the effect of L-dopa and phenoxybenzamine on the rigidity of Parkinson's disease
