Granulocyte chemotaxis: multiple assay screening using a raft technique.

AUTOR(ES)

Jayaswal, U

RESUMO

The assessment of granulocyte chemotaxis is complicated by the difficulty of precisely reproducing results in serial estimations and deciding on the best end point which would reflect most accurately the degree of travel taken by the cells under observation. The methods in use are generally based on the Boyden chamber, following this, we have further developed the principle of the "raft" technique of chamber based migration. In order to overcome the problems associated with reproducibility of results when performing multiple assays of chemotaxis, especially when sera of widely differing activity are encountered in the screening procedure, we have used a "batching" system and a simple method of presenting the results so that they are comparable.

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