Growth and cytopathology of Mycoplasma synoviae in chicken embryo cell cultures.
AUTOR(ES)
Aldridge, K E
RESUMO
Mycoplasma synoviae was tested for its ability to grow and induce cytopathogenic changes in chicken embryo cell cultures. M. synoviae grew to high titers by day 5 in the presence of chick cells, but showed no growth in the tissue culture medium alone even though it was enriched with nicotinamide adenine dinucleotide and swine serum. Infected chick cell cultures showed a progressive cytoplasmic degeneration on successive days of examination. Early changes involved cytoplasmic granularity and mild vacuolation. On the last day of examination the cytoplasm of most cells was completely degenerated and some showed nuclear degeneration. M. synoviae was shown to be cytophilic for the chick cell membranes where the mycoplasmas reproduced and formed microcolonies which, on successive days, increased in size. The attachment site on the chick cell membrane was shown to be neuraminidase sensitive.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=415267Documentos Relacionados
- Pathogenicity and cytadherence of Mycoplasma imitans in chicken and duck embryo tracheal organ cultures.
- Growth and Pathogenesis of Mycoplasma mycoides var. capri in Chicken Embryo Tracheal Organ Cultures
- Immunofluorescence and electron microscopy of the attachment of Mycoplasma synoviae to chicken embryo fibroblasts.
- Opioid peptides activate phospholipase D and protein kinase C-epsilon in chicken embryo neuron cultures.
- Large-Quantity Production of Chicken Embryo Tracheal Organ Cultures and Use in Virus and Mycoplasma Studies