Growth and subculture of pathogenic T. pallidum (Nichols strain) in BHK-21 cultured tissue cells.
AUTOR(ES)
Jones, R H
RESUMO
The growth and nine subcultivations of the experimental Nichols strain of pathogenic Treponema pallidum were successfully accomplished in cultured baby hamster kidney tissue cells (BHK-21) using serum-free media. The number (motile and non-motile cells occurring extracellulary) of cell generations generally increased with each subcultivation, the largest increases (greater than 3-0) occurring in subcultures 4, 5, 7, 8, and 9. Also, the number of motile cells decreased with each subcultivation. Virulent organisms were demonstrated in subcultures 1, 4, 5, 7, and 8, and their numbers estimated with the use of standards established by the animal inoculation titration of counted numbers of organisms freshly harvested from experimental infections; the estimated number of virulent organisms was higher than or equal to the counted cultivated treponemes injected, which may be attributable to uncounted virulent spirochaetes occurring intracellularly.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1045204Documentos Relacionados
- Scanning electron microscopy of Treponema pallidum (Nichols strain) attached to cultured mammalian cells.
- Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.
- Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.
- Treponema pallidum (Nichols Strain) in Tissue Cultures: Cellular Attachment, Entry, and Survival
- Mucopolysaccharide Material Resulting from the Interaction of Treponema pallidum (Nichols Strain) with Cultured Mammalian Cells
