Growth of Legionella pneumophila in continuous culture.
AUTOR(ES)
Berg, J D
RESUMO
A method was developed to grow Legionella pneumophila in continuous culture. A chemostat was used to simulate nutrient-limited, submaximal growth in the natural environmental and to provide a precisely controlled growth regimen. Cultures grew under forced aeration under conditions yielding up to 38% saturation of dissolved oxygen; supplemental CO2 (5%) at the same gas flow rates as ambient air had no effect on culture growth. Pleomorphism was observed during growth under all conditions. Pigment was produced only at D less than 0.03 h-1. Catalase was produced at higher growth rates but not at higher temperatures. The pathogenicity was unaffected by altering either the growth rate or the growth temperature.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=241762Documentos Relacionados
- Influence of iron-limited continuous culture on physiology and virulence of Legionella pneumophila.
- Cultivation of Ureaplasma urealyticum in continuous culture.
- Temperature-dependent growth kinetics of Escherichia coli ML 30 in glucose-limited continuous culture.
- Pure spherules of Coccidioides immitis in continuous culture.
- Response of Rhodopseudomonas capsulata to illumination and growth rate in a light-limited continuous culture.