Growth, Respiration, and Polypeptide Patterns of Bradyrhizobium sp. (Arachis) Strain 3G4b20 from Succinate- or Oxygen-Limited Continuous Cultures †
AUTOR(ES)
Allen, George C.
RESUMO
Succinate- or oxygen-limited continuous cultures were used to study the influences of different concentrations of dissolved oxygen and ammonia on the growth, respiration, and polypeptide patterns of Bradyrhizobium sp. (Arachis) strain 3G4b20. During succinate-limited growth, molar growth yields on succinate (Ysucc) ranged from 38.9 to 44.4 g (dry weight) of cells mol of succinate−1 and were not greatly influenced by changes in dilution rates or changes in the oxygen concentrations that we tested. Succinate, malate, and fumarate induced the highest rates of oxygen uptake in all of the steady states in which the supply rates of (NH4)2SO4 ranged between 322 and 976 μmol h−1. However, the amino acids aspartate, asparagine, and glutamate could also be used as respiratory substrates, especially when the (NH4)2SO4 supply rate was decreased to 29 μmol h−1. Glutamine-dependent respiration was seen only when the (NH4)2SO4 supply rate was 29 μmol h−1 and thus appears to be under tight ammonia control. Nitrogenase activity was detected only when the culture was switched from a succinate-limited steady state to an oxygen-limited steady state. Comparison of major silver-stained proteins from three steady states by two-dimensional gel electrophoresis revealed that nearly 60% were affected by oxygen and 24% were affected by ammonia. These data are consistent with reports that oxygen has a major regulatory role over developmental processes in Rhizobium sp. and Bradyrhizobium sp.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=184338Documentos Relacionados
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