H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells.
AUTOR(ES)
Bogachev, A V
RESUMO
Anaerobically grown Escherichia coli cells were shown to acidify the reaction medium in response to oxygen or dimethyl sulfoxide (DMSO) pulses, with the H+/e- stoichiometry being close to 2.5 and 1.5, respectively. In the presence of the NADH dehydrogenase I (NDH-I) inhibitor 8-methyl-N-vanillyl-6-nonenamide (capsaicin) or in mutants lacking NDH-I, this ratio decreased to 1 for O2 and to 0 for DMSO. These data suggest that (i) the H+/e- stoichiometry for E. coli NDH-I is at least 1.5 and (ii) the DMSO reductase does not generate a proton motive force.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=178495Documentos Relacionados
- Dimethyl sulfoxide reductase activity by anaerobically grown Escherichia coli HB101.
- Proton translocation coupled to dimethyl sulfoxide reduction in anaerobically grown Escherichia coli HB101.
- Molecular cloning and expression of the Escherichia coli dimethyl sulfoxide reductase operon.
- Organization of dimethyl sulfoxide reductase in the plasma membrane of Escherichia coli.
- Energy Supply for Active Transport in Anaerobically Grown Escherichia coli
