Hapten-Conjugated Antibodies and Visual Markers Used to Label Cell-Surface Antigens for Electron Microscopy: An Approach to Double Labeling
AUTOR(ES)
Lamm, Michael E.
RESUMO
Haptens can be coupled to mouse immunoglobulin with retention of antibody specificity. Such hapten-coupled alloantibody, when bound to cells, can be bridged to an electron microscopic marker in two ways: (i) by a hybrid antibody, i.e., anti-hapten:anti-marker, and (ii) by untreated bivalent anti-hapten antibody if the marker is also haptenized. Through the use of immunologically non-crossreactive haptens and markers it should be possible to localize multiple, different antigens on the surface of an individual cell. Double labeling has been achieved with the first method.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=389859Documentos Relacionados
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