Headgroup conformation and lipid--cholesterol association in phosphatidylcholine vesicles: a 31P(1H) nuclear Overhauser effect study.
AUTOR(ES)
Yeagle, P L
RESUMO
The nuclear Overhauser effect has been observed in the nuclear magnetic resonance spectra of 31P. The information content of the nuclear Overhauser effect has been applied to the structure and dynamic properties of phosphatidylcholine vesicles. In the vesicles only 1/3 of the theoretical maximum nuclear Overhauser effect enhancement is observed. This result is accounted for by dipolar interactions between the N-methyl protons and the phosphate of phosphatidylcholine, and a correlation time for internal motion of 1.4 X 10(-9) sec. Addition of up to 30% cholesterol does not change the nuclear Overhauser effect enhancement or spin-lattice relaxation time of the vesicles. It is argued that the OH group of cholesterol is hydrogen bonded to the ester carbonyl oxygen of the phosphatidylcholine molecules.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433017Documentos Relacionados
- Conformation of gramicidin A channel in phospholipid vesicles: a 13C and 19F nuclear magnetic resonance study.
- Effects of pH and cholesterol on DMPA membranes: a solid state 2H- and 31P-NMR study.
- Self-association of the polyene antibiotic nystatin in dipalmitoylphosphatidylcholine vesicles: a time-resolved fluorescence study.
- Solution conformation of gramicidin S: An intramolecular nuclear Overhauser effect study
- Interaction of vesicular stomatitis virus with lipid vesicles: depletion of cholesterol and effect on virion membrane fluidity and infectivity.
