Hepatitis B virus X protein interacts with a probable cellular DNA repair protein.
AUTOR(ES)
Lee, T H
RESUMO
The mechanism of action of hepatitis B virus (HBV) X protein in transcriptional transactivation and in tumorigenesis remains obscure. We have used the yeast two-hybrid system to identify a cellular protein that can interact with HBV X protein. This protein, designated X-associated protein 1 (XAP-1), is a human homolog of the UV-damaged DNA-binding protein (UV-DDB) recovered from a monkey cell cDNA library. UV-DDB is presumed to be involved in DNA repair. The interaction between X protein and XAP-1 protein was verified by immunoprecipitation of yeast cell lysates expressing both proteins and by in vitro mixing with X protein expressed as a glutathione S-transferase fusion protein and XAP-1 protein either in HeLa cell extracts or synthesized by in vitro translation. We speculate that the interaction of X protein with a DNA repair protein may recruit cellular proteins to repair the partially double-stranded HBV genome or may modify cellular transcription processes. An effect on the cellular DNA repair system may explain a cofactor role for HBV in liver cancer development.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=188683Documentos Relacionados
- Hepatitis B Virus X Protein Interferes with Cellular DNA Repair
- Hepatitis B virus transactivator protein X interacts with the TATA-binding protein.
- Proteasome complex as a potential cellular target of hepatitis B virus X protein.
- Hepatitis C Virus Core Protein Interacts with Cellular Putative RNA Helicase
- Mapping of B-cell epitopes of the human hepatitis B virus X protein.
