Heterogeneity of biologically active deadenylated protamine mRNA components isolated from rainbow trout testes.
AUTOR(ES)
Gedamu, L
RESUMO
Poly(A)+ protamine mRNA's were isolated from rainbow trout testes and deadenylated by treatment with calf thymus RNase H. Four subcomponents of deadenylated PmRNA (PmRNA1-4) were purified by electrophoresis on a 6% polyacrylamide gel in 8 M urea. Translation of each PmRNA subcomponent in the wheat germ S-30 cell-free system showed that all subcomponents are biologically active but each codes for two or more protamine polypeptides suggesting molecular heterogeneity. However, the deadenylated mRNA's can be categorized into two groups based on the spectrum of protamines whose synthesis they stimulate.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327964Documentos Relacionados
- Molecular cloning of three major sequence species from Rainbow trout protamine mRNA.
- Sequences of large T1 ribonuclease-resistant oligoribonucleotides from protamine mRNA: the overall architecture of protamine mRNA.
- Sequence homologies in the protamine gene family of rainbow trout.
- Alternative mRNA Splicing Produces a Novel Biologically Active Short Isoform of PGC-1α*
- Molecular analysis of the protamine multi-gene family in rainbow trout testis.