High-Level Overproduction of His-Tagged Tth DNA Polymerase in Thermus thermophilus
AUTOR(ES)
Moreno, Renata
FONTE
American Society for Microbiology
RESUMO
A new plasmid for the overexpression of His-tagged thermozymes in Thermus thermophilus was developed. With this plasmid, soluble and active histidine-tagged DNA polymerase from T. thermophilus was overproduced in larger amounts in the thermophile than in Escherichia coli. The protein purified from the thermophile was active in PCR.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=544263Documentos Relacionados
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