Highly parallel measurements of interaction kinetic constants with a microfabricated optomechanical device
AUTOR(ES)
Bates, Steven R.
FONTE
American Institute of Physics
RESUMO
We used mechanical trapping of molecular interactions to demonstrate a highly parallel approach to measure the kinetics of biomolecular interactions. This approach consumes 25 fmol of material per measurement and permits 320 measurements in a single experiment. We measured association and dissociation curves for the interactions of 6-His and T7 epitope tags with their antibodies, from which we determined the off rates, on rates, and dissociation constants.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2749446Documentos Relacionados
- DNA typing in thirty seconds with a microfabricated device
- KINETIC CONSTANTS OF THE INTERACTION BETWEEN MYOSIN AND ADENOSINETRIPHOSPHATE*
- Kinetic Constants Determined from Membrane Transport Measurements: Carbonic Anhydrase Activity at High Concentrations
- A microfabricated device for sizing and sorting DNA molecules
- Neutron correlations with electrical measurements in a Plasma Focus device
