Histologia, imunohistoquimica e ultra-estrutura do epitelio da uretra pelvica e peniana nas linhagens de ratos albinos UChA e UChB, bebedoras voluntarias de etanol

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

The extreme use of ethanol causes significant alterations in the urogenital tract, however papers relating the ethanol chronic consumption to the urethral morphology are unknown. The objective of the present paper was to describe the histology, histochemistry, immunohistochemistry and ultrastructure of the urethral epithelium in an ethanol-drinking rat strain (UChA and UChB). Seventy-four adult male rats of the following groups were used: (1) UChA rats of low voluntary consumption of ethanol 10% (water + ethanol = 5.86g/Kg/day), (2) UChB rats of high voluntary consumption of ethanol 10% (water + ethanol = 7.16g/Kg/day) and (3) Wistar of voluntary water consumption. Conventional techniques of light, transmission electron microscopy and morphometrics were utilized. The pelvic urethral epithelium presented three cellular types: I, II and neuroendocrine (NE). The type I presented rich cytoplasmic matrix in free ribosomes, rough endoplasmic reticulum and mitochondria. The type II with similar characteristics to the described to the type I, increased of Golgi complex and cellular apex with microvilli. The I and II cells presented lipid drops near to the nucleus. The NE cells presented argyrophil and serotoninpositive. The penile urethra presented four cellular types: basal, intermediate, superficial and NE. The basal cell was characterized by nucleus and cytoplasmic matrix electron dense and presence of intermediate cytoplasmic filaments. The intermediate cell presented clear cytoplasmic matrix, Golgi complex and rough endoplasmic reticulum in concentric whorls. The superficial cell presented lipid drops and cellular apex with short microvilli. In the samples observed of the penile urethra, there isn t positive reaction for histochemistry with silver or for immunohistochemistry with serotonin. Ultrastructurally, NE presented rich cytoplasmic matrix in granules. The propria lamina of the pelvic and penile epithelium, it observed myelinated and nonmyelinated nerve fibers. It concludes that the ethanol chronic consumption induces morphological and physiological alterations in the UCh rats urethral epithelium; the immunohistochemistry and ultrastructure of the control rat urethral NE cells and UChA and UChB groups are similar to the other APUD system cellular types; the structural association between the urethral epithelium and afferent nervous fibers influences the NE cells and the other urethral epithelium cells types through paracrine action and the UCh rats presented adaptations to the ethanol chronic ingestion.

ASSUNTO(S)

morfologia rato alcoolismo

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