Histological and radiographical analysis of the repair of rabbit femur deffects filled with bovine bone organic cancellous matrix in blocks or cortical matrix in micro granules / Análises microscópica e radiográfica do reparo de defeitos confeccionados em fêmures de coelhos preenchidos com matriz óssea bovina medular em bloco ou cortical em microgrânulos

AUTOR(ES)

Luciana Viti Betti

DATA DE PUBLICAÇÃO

2004

RESUMO

The aim of this work was to verify the osseous repair in non through-andthrough defects treated with blocks of lyophilized bovine bone organic matrix of cancellous origin or micro granules (0.25-1.0 mm) of lyophilized bovine bone organic matrix of cortical origin (both materials Gen-Ox®- Baumer SA- Brazil). An osseous defect, 6mm in diameter by 8mm in depth, was created in both distal femoral epiphyses of male New Zealand White rabbits using a trephine bur. Fifteen defects were grafted with cancellous blocks (experimental group I), fifteen with cortical micro granules (experimental group II) and fifteen were filled with blood clot (control). The rabbits of each group were sacrificed 30, 90 and 180 days after surgeries. Thereafter, the epiphyses were collected and fixed. Radiographs were taken using the Digora digital radiographic system and conventional periapical films. The radiographic digital images (positive and negative) and the conventional radiographs were evaluated using scores by two examiners. After this, the epiphyses were demineralized with EDTA and embedded in paraffin. Semi-serial 6-µm thick sections were obtained, stained with hematoxylin and eosin and Masson Trichromic and examined under a light microscope. After 30 days the experimental group II presented some particles of bone matrix in resorption by mononuclear cells and a larger amount of primary woven bone than did the other groups. Both experimental groups showed total or partial closure by woven bone while the control group exhibited migration of the connective tissue into the defect area. After 90 days the experimental and control groups showed intense remodelation of neoformed woven bone that was replaced by lamellar bone. Generally, after 180 days both experimental and control groups exhibited a similar pattern of repair. In the radiographic analysis, neither intra-examiners significant differences nor interexaminers significant differences were observed. Also no significant differences among the groups in all evaluated periods were observed (p>0.05). The osseous repair evaluated by digital and conventional radiographs was similar in both experimental groups and control groups in the periods of 30, 90 and 180 days post-surgery. Although the bone formation appeared similar in the experimental and control groups after 180 days by the histological analysis and in all periods by radiographic analysis, the use of lyophilized bovine bone organic matrix in blocks or micro granules avoided the migration of connective tissue into the defect area in the initial phases of the repair.

ASSUNTO(S)

fêmur de animal coelhos medula óssea de animal reparo ósseo

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