Homotypic interaction and multimerization of nucleocapsid protein of tomato spotted wilt tospovirus: Identification and characterization of two interacting domains
AUTOR(ES)
Uhrig, J. F.
FONTE
The National Academy of Sciences
RESUMO
The nucleocapsid protein (N) of tomato spotted wilt tospovirus (TSWV) plays a central role in the viral life cycle. With the aid of the yeast two-hybrid system and surface plasmon resonance analysis, homotypic interaction and multimerization of the N protein was detected. Analysis of deletion mutants identified two binding regions in the protein, located at the N terminus (amino acids 1–39) and the C terminus (amino acids 233–248), respectively, implying a “head-to-tail” interaction of the N terminus with the C terminus to form a multimeric chain. Further characterization of the binding domains was performed by site-directed mutagenesis. Two phenylalanines (F242 and F246) highly conserved in the N proteins within the Tospovirus genus were shown to play a crucial role in the interaction.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=15092Documentos Relacionados
- The movement protein NSm of tomato spotted wilt tospovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting plant proteins
- The Plant Virus Tomato Spotted Wilt Tospovirus Activates the Immune System of Its Main Insect Vector, Frankliniella occidentalis
- Expression and Characterization of a Soluble Form of Tomato Spotted Wilt Virus Glycoprotein GN
- Tomato Spotted Wilt Virus Particle Morphogenesis in Plant Cells
- Viral genetic determinants for thrips transmission of Tomato spotted wilt virus
