Host sequences flanking the HIV provirus.
AUTOR(ES)
Vincent, K A
RESUMO
A conserved property of retroviral proviruses is the presence of a direct repeat in the host DNA immediately flanking the viral sequence; each virus generates a repeat with a characteristic length. By sequencing the viral/host DNA junctions from five HIV-1 proviral clones, we have confirmed that integration of HIV results in the generation of a five basepair direct repeat. A target sequence in uninfected host DNA was analyzed to establish that the five basepair sequence flanking the provirus was present only once prior to integration. Of the five proviruses examined, two were found to have integrated in known repetitive sequence elements of the human genome; one in a Line-1 element and a second in satellite DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=332403Documentos Relacionados
- Host Sequences Flanking the Human T-Cell Leukemia Virus Type 1 Provirus In Vivo
- Host restriction of Friend leukemia virus: synthesis and integration of the provirus.
- Murine leukemia virus: detection of unintegrated double-stranded DNA forms of the provirus.
- Human cells infected with retrovirus vectors acquire an endogenous murine provirus.
- Nucleotide sequence and distinctive characteristics of the env gene of endogenous feline leukemia provirus.