How Does Replication-Associated Mutational Pressure Influence Amino Acid Composition of Proteins?

AUTOR(ES)

Mackiewicz, Pawel

FONTE

Cold Spring Harbor Laboratory Press

RESUMO

We have performed detrended DNA walks on whole prokaryotic genomes, on noncoding sequences and, separately, on each position in codons of coding sequences. Our method enables us to distinguish between the mutational pressure associated with replication and the mutational pressure associated with transcription and other mechanisms that introduce asymmetry into prokaryotic chromosomes. In many prokaryotic genomes, each component of mutational pressure affects coding sequences not only in silent positions but also in positions in which changes cause amino acid substitutions in coded proteins. Asymmetry in the silent positions of codons differentiates the rate of translation of mRNA produced from leading and lagging strands. Asymmetry in the amino acid composition of proteins resulting from replication-associated mutational pressure also corresponds to leading and lagging roles of DNA strands, whereas asymmetry connected with transcription and coding function corresponds to the distance of genes from the origin or terminus of chromosome replication.

Documentos Relacionados

• Replication-associated strand asymmetries in mammalian genomes: Toward detection of replication origins
• In Situ Localization and Tissue Distribution of the Replication-Associated Proteins of Cucumber Mosaic Virus in Tobacco and Cucumber
• The Werner Syndrome Protein Functions in Repair of Cr(VI)-Induced Replication-Associated DNA Damage
• Identification of the replication-associated protein binding domain within the intergenic region of tomato leaf curl geminivirus.
• Can amino acid requirements for nutritional maintenance in adult humans be approximated from the amino acid composition of body mixed proteins?