Human CD34+ cells differentiate into microglia and express recombinant therapeutic protein
AUTOR(ES)
Asheuer, Muriel
FONTE
National Academy of Sciences
RESUMO
In rodents, bone marrow-derived cells enter the brain during adult life. Allogeneic bone marrow transplantation is used to treat genetic CNS diseases, but the fate of human bone marrow and CD34+ cells within the brain remains to be elucidated. The present study demonstrates that cells derived from human CD34+ cells, isolated from either cord blood or peripheral blood, migrate into the brain after infusion into nonobese diabetic/severe combined immunodeficient mice. Both types of CD34+-derived cells differentiate into perivascular and ramified microglia. The lentiviral transfer of genes into CD34+ cells before infusion does not modify the differentiation of human CD34+ cells into microglia, allowing new transgenic proteins to be expressed in these cells. The transplantation of CD34+ cells could thus be used for the treatment of CNS diseases.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=373501Documentos Relacionados
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