Human DNA (cytosine-5)methyltransferase selectively methylates duplex DNA containing mispairs.
AUTOR(ES)
Smith, S S
RESUMO
The presence of the C.C mispair in a defined duplex oligodeoxynucleotide enhanced its capacity to serve as a substrate for highly purified human DNA methyltransferase. Analysis of tritiated reaction products showed that the C.C mispair acted as a "methylation acceptor" in that it was itself rapidly methylated. The m5C.G base pair also enhanced the capacity of the oligodeoxynucleotide to serve as a substrate for the enzyme. However, this complementary base pair was found to act as a "methylation director". That is, the presence of the m5C in one strand induced the enzyme to rapidly methylate at the cytosine residue on the opposite strand in an adjacent C.G base pair.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=306183Documentos Relacionados
- Structural features and hydration of a dodecamer duplex containing two C.A mispairs.
- Covalent bond formation between a DNA-cytosine methyltransferase and DNA containing 5-azacytosine.
- Two major forms of DNA (cytosine-5) methyltransferase in human somatic tissues
- Methylation of slipped duplexes, snapbacks and cruciforms by human DNA(cytosine-5)methyltransferase.
- Methylation inhibitors can increase the rate of cytosine deamination by (cytosine-5)-DNA methyltransferase.
