Human immune interferon: induction in lymphoid cells by a calcium ionophore.

AUTOR(ES)

Dianzani, F

RESUMO

Human lymphocyte cultures produced interferon after treatment with the calcium ionophore A23187, but not after treatment with potassium ionophore nonactin. Interferon production was detectable between 3 and 6 h after ionophore treatment and reached the maximum level between 12 and 24 h. Ionophore-induced interferon appeared to be immune interferon on the basis of acid lability, lack of neutralization by antibody to leukocyte interferon, slow kinetics of activation of the antiviral state, and molecular characterization by isoelectrofocusing.

Documentos Relacionados

• Enzymatic induction of interferon production by galactose oxidase treatment of human lymphoid cells.
• c-fos sequence necessary for basal expression and induction by epidermal growth factor, 12-O-tetradecanoyl phorbol-13-acetate and the calcium ionophore.
• Immune interferon production by lymphoid cells: role in the inhibition of herpesviruses.
• Interferon: purification and initial characterization from human diploid cells.
• Cellular Alteration by Interferon: a Virus-Free System for Assaying Interferon