Human immunoglobulin kappa light chain genes of subgroups II and III.
AUTOR(ES)
Klobeck, H G
RESUMO
The first complete sequences of functionally rearranged VK genes (abbreviations ref. 1) of subgroups II and III are reported. The genes have been cloned from lymphoid cell lines synthesizing KII or KIII light chains as evidenced from immunochemical analyses with anti-VK subgroup-specific antisera. These data, together with the sequence of a KIV gene (described in the accompanying paper) and those of previously published KI genes make possible a comparison of genes representative of the four known V region subgroups of human K light chains. The VKII gene is distinguished from the VKI, VKIII, and VKIV genes by a much longer intron within the leader sequence: 426 bp vs ca. 120-220 bp. Blot hybridization experiments with human DNA digests using probes from the KII and KIII genes and from the respective upstream regions help to define subgroup specific probes and hybridization conditions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=321973Documentos Relacionados
- Two rearranged immunoglobulin kappa light chain genes in one mouse myeloma.
- Cloning of immunoglobulin kappa light chain genes from mouse liver and myeloma MOPC 173
- Immunoglobulin genes of the kappa light chain type from two human lymphoid cell lines are closely related.
- DNase I-hypersensitive sites in the chromatin of immunoglobulin kappa light chain genes.
- Correlation between DNase I hypersensitive sites and putative regulatory sequences in human immunoglobulin genes of the kappa light chain type.