Hybridization of pigeon globin messenger RNA with complementary DNA synthesized in vitro by reverse transcription: influence of the homopolymeric regions.
AUTOR(ES)
Gasaryan, K G
RESUMO
The kinetics of hybridization of pigeon globin messenger RNA with complementary cDNA synthesized by means of AMV reverse transcriptase is complex. Addition of poly A or poly U in excess to the reaction mixture normalized the kinetics. It is concluded that association of the complementary homopolymeric regions of mRNA and cDNA accelerates the complex formation between heteropolymeric sequences in a fraction of the molecules.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=343506Documentos Relacionados
- Nucleotide Sequence Analysis of RNA Synthesized from Rabbit Globin Complementary DNA
- Decreased Globin Messenger RNA in Thalassemia Detected by Molecular Hybridization
- In Vitro Synthesis of DNA Complementary to Purified Rabbit Globin mRNA
- Segments of Influenza Virus Complementary RNA Synthesized In Vitro
- Enzymatic synthesis of DNA complementary to mitochondrial mRNA via reverse transcription.
