Hydrogen peroxide production, chemiluminescence, and the respiratory burst of fertilization: Interrelated events in early sea urchin development

AUTOR(ES)

Foerder, Charles A.

RESUMO

After fertilization of the sea urchin, Strongyl-ocentrotus purpuratus, a crosslinked fertilization membrane is formed; the crosslinks (dityrosine residues) are synthesized in a reaction catalyzed by an ovoperoxidase that is released from the cortical granules during fertilization. The substrate for ovoperoxidase activity, hydrogen peroxide, is generated by the egg coincident with the “respiratory burst” that follows parthenogenetic activation by the divalent ionophore A23187 or fertilization. This burst of oxygen consumption may be almost quantitatively accounted for by hydrogen peroxide evolution, as measured by the peroxidase-catalyzed quenching of scopoletin fluorescence. Neither the burst of oxygen consumption nor hydrogen peroxide production occurs when the inhibitor of cortical granule discharge, procaine, is present at fertilization.

Documentos Relacionados

• Endotoxin in vitro interactions with human neutrophils: depression of chemiluminescence, oxygen consumption, superoxide production, and killing.
• Temperature, but not pH, compromises sea urchin fertilization and early development under near-future climate change scenarios
• The nature of messenger RNA in the early stages of sea urchin development.
• Respiratory burst oxidase of fertilization.
• Dynamics of the Endoplasmic Reticulum and Golgi Apparatus during Early Sea Urchin Development