Hydrogen Peroxide-Releasing Function of Chemically Elicited and Immunologically Activated Macrophages: Differential Response to Wheat Germ Lectin and Concanavalin A

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Various types of mouse peritoneal macrophages were studied for H2O2 release in the presence of wheat germ lectin or phorbol myristate acetate. Macrophages elicited 3 days before harvest by a single injection of thioglycolate, zymosan A, or a streptococcal preparation (OK-432) were highly responsive to wheat germ lectin, resulting in a marked increase in H2O2 release. However, immunologically activated macrophages induced by double injections of live and heat-killed BCG at 15 and 3 days before harvest or by double injections of zymosan A or OK-432 at 20 and 3 days before harvest did not show any significant response to wheat germ lectin. On the other hand, all macrophages tested responded well to phorbol myristate acetate by augmentation of H2O2 release. Concanavalin A inhibited wheat germ lectin- and phorbol myristate acetate-triggered H2O2 release from all types of macrophages, but inhibition was much more marked in the case of wheat germ lectin-stimulated H2O2 release. Succinylated concanavalin A (divalent concanavalin A) showed only slight suppressive action against macrophage H2O2 release, and prostaglandin E1 and dibutyryl cyclic adenosine 3′, 5′-monophosphate caused depression of H2O2 release from OK-432-induced macrophages.

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