Hyperactive transposase mutants of the Himar1 mariner transposon
AUTOR(ES)
Lampe, David J.
FONTE
The National Academy of Sciences
RESUMO
Mariner-family transposable elements are active in a wide variety of organisms and are becoming increasingly important genetic tools in species lacking sophisticated genetics. The Himar1 element, isolated from the horn fly, Haematobia irritans, is active in Escherichia coli when expressed appropriately. We used this fact to devise a genetic screen for hyperactive mutants of Himar1 transposase that enhance overall transposition from ≈4- to 50-fold as measured in an E. coli assay. Purified mutant transposases retain their hyperactivity, although to a lesser degree, in an in vitro transposition assay. Mutants like those described herein should enable sophisticated analysis of the biochemistry of mariner transposition and should improve the use of these elements as genetic tools, both in vivo and in vitro.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=18050Documentos Relacionados
- The Himar1 mariner transposase cloned in a recombinant adenovirus vector is functional in mammalian cells.
- Mutagenesis of Neisseria meningitidis by In Vitro Transposition of Himar1 mariner
- Phenotypic Mutants of the Intracellular Actinomycete Rhodococcus equi Created by In Vivo Himar1 Transposon Mutagenesis
- In vivo transposon mutagenesis of the methanogenic archaeon Methanosarcina acetivorans C2A using a modified version of the insect mariner-family transposable element Himar1
- Subunit Interactions in the Mariner Transposase