Identification and expression of a copy number control gene in the IncFIII hemolytic plasmid pSU316.

AUTOR(ES)

Andrés, I

RESUMO

A DNA fragment carrying both the IncFIII determinant and a copy number control gene of the hemolytic plasmid pSU316 has been cloned in pBR322. Deletion derivatives of the hybrid plasmid generated by Bal 31 digestion, which no longer exhibit the IncFIII phenotype, fall into two complementation groups when tested against a pSU316 miniplasmid derivative. Type 1 mutants exhibit the copy number control (Cop+) phenotype whereas type 2 mutants do not. Restriction analysis of type 1 and type 2 mutants allowed us to locate the cop gene of pSU316 in a 700-base-pair fragment adjacent to the IncFIII determinant. Plasmid expression in a minicell system suggests that the product of the cop gene of pSU316 could be a 13,000-dalton protein.

Documentos Relacionados

• Nucleotide sequence and expression of the copy number control gene (cop) of the incFVII plasmid pSU233.
• Nucleotide sequence of the oriT-traM-finP region of the haemolytic plasmid pSU316: comparison to F.
• Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194.
• Convergent transcription interferes with expression of the copy number control gene, copA, from plasmid R1
• Purification and characterization of RepA, a protein involved in the copy number control of plasmid pLS1.