Identification and genetic mapping of a herpes simplex virus capsid protein that binds DNA.
AUTOR(ES)
Braun, D K
RESUMO
Herpes simplex virus virion protein 19C (VP19C) is a constituent of both unenveloped (nuclear) and enveloped (cytoplasmic) capsids. In this paper we report that 32P-labeled DNA, either supercoiled or linear double stranded, efficiently bound to VP19C electrically transferred from denaturing polyacrylamide gels containing electrophoretically separated proteins from purified capsids. Analyses of the polypeptides specified by herpes simplex virus type 1 X herpes simplex type 2 recombinants with respect to electrophoretic mobility and binding of 32P-labeled DNA indicate that VP19C maps at the same location as infected cell polypeptide 32 and is derived from it.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=255696Documentos Relacionados
- Electron microscopic mapping of proteins bound to herpes simplex virus DNA.
- Identification of a new glycoprotein of herpes simplex virus type 1 and genetic mapping of the gene that codes for it.
- Role of the UL25 Gene Product in Packaging DNA into the Herpes Simplex Virus Capsid: Location of UL25 Product in the Capsid and Demonstration that It Binds DNA
- Structure of replicating herpes simplex virus DNA.
- A protein that preferentially binds Drosophila satellite DNA.