Identification and purification of a factor that binds to the Mlu I cell cycle box of yeast DNA replication genes.
AUTOR(ES)
Verma, R
RESUMO
In Saccharomyces cerevisiae, the genes encoding at least 10 enzymes involved in DNA replication are periodically expressed in the late G1 and S phases of the cell cycle. All of these genes have one copy or more of the sequence ACGCGT, which conforms to the recognition site for the Mlu I restriction endonuclease. For the CDC21, CDC9, and POL1 genes, the Mlu I site has been shown to be absolutely required for periodic transcription. Using nuclear extracts fractionated by conventional and oligonucleotide affinity chromatography, we have purified a 17-kDa protein that recognizes the Mlu I motif. Synthetic oligonucleotides containing mutated Mlu I sites do not bind the protein. In contrast, synthetic oligonucleotides derived from the CDC2, CDC6, and CDC21 genes, which are expressed with the same timing as POL1, bind purified protein efficiently.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=52252Documentos Relacionados
- Regulation of the yeast DNA replication genes through the Mlu I cell cycle box is dependent on SWI6.
- Replication protein A binds to regulatory elements in yeast DNA repair and DNA metabolism genes.
- Purification and characterization of an erythroid cell-specific factor that binds the murine alpha- and beta-globin genes.
- Purification of a yeast protein that binds to origins of DNA replication and a transcriptional silencer.
- Identification of a 150-kilodalton polypeptide that copurifies with yeast TFIIIC and binds specifically to tRNA genes.