Identification, mapping, and cloning of the gene encoding cyanase in Escherichia coli K-12.

AUTOR(ES)

Sung, Y C

RESUMO

The gene in Escherichia coli for cyanase, designated cynS, was localized to a BglII restriction site approximately 1.7 kilobases from the lacA end of the lac operon. The gene was cloned into the pUC13 vector. Maxicell analysis of plasmid-encoded proteins confirmed that the BglII site is in the region encoding the structural gene for cyanase. Cyanase-deficient strains had increased sensitivity to cyanate and were not able to use cyanate as a nitrogen source.

Documentos Relacionados

• Cloning, mapping, and expression of the fumarase gene of Escherichia coli K-12.
• Cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12.
• Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12.
• Cloning, expression, and mapping of the Aeromonas hydrophila aerolysin gene determinant in Escherichia coli K-12.
• Isolation, cloning, mapping, and nucleotide sequencing of the gene encoding flavodoxin in Escherichia coli.