Identification of a novel TA-rich DNA binding protein that recognizes a TATA sequence within the brain creatine kinase promoter.
AUTOR(ES)
Hobson, G M
RESUMO
The rat brain creatine kinase gene possesses a structurally complex promoter with multiple potential regulatory elements. Two CCAAT sequences, a TATAAATA sequence and a TTAA sequence are found within the first one hundred base pairs. We present evidence that favors the allocation of the downstream TTAA sequence as the potential TATA box. We show that the CCAAT sequences and the upstream TATAAATA sequence are binding sites for potential regulatory factors and that sequences in this region are capable of regulating expression from the downstream TTAA sequence. We suggest that the protein that binds to the upstream TATAAATA sequence is not a classical TFIID factor but rather may serve to block the binding of TFIID and/or to promote transcription from the downstream start site. We have been able to define conditions in vitro under which binding to this upstream TATAAATA sequence does not occur. Under these conditions we are able to detect transcription from both potential TATA sequences, a situation which we have been unable to detect in vivo. Our experiments suggest the existence in HeLa and brain nuclei of a protein that recognizes the concensus TATAAATA sequence, that is distinct from TFIID, and that may function in part to deny access of TFIID to this potential promoter element.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=338643Documentos Relacionados
- Brain and muscle creatine kinase genes contain common TA-rich recognition protein-binding regulatory elements.
- Identification of a DNA binding protein that recognizes the nonamer recombinational signal sequence of immunoglobulin genes.
- The complete sequence of soybean chlorotic mottle virus DNA and the identification of a novel promoter.
- A sequence-specific single-strand DNA binding protein that contacts repressor sequences in the human GM-CSF promoter.
- Identification of cis-acting regulatory elements in the promoter region of the rat brain creatine kinase gene.
