Identification of an interactor of cadmium ion-induced glycine-rich protein involved in regulation of callose levels in plant vasculature

AUTOR(ES)

Ueki, Shoko

FONTE

National Academy of Sciences

RESUMO

Cadmium-induced glycine-rich protein (cdiGRP) is a cell wall-associated factor that increases callose levels in plant vasculature. To better understand the cdiGRP/callose regulation system, we identified a tobacco protein, GrIP (cdiGRP-interacting protein, GrIP), that associates with cdiGRP and localizes at the plant cell wall. Constitutive overexpression of GrIP enhanced the accumulation of the cdiGRP protein and callose in vasculature-associated cells with or without treatment with cadmium ions. That GrIP gene expression was not affected by cadmium ions indicated that GrIP does not directly modulate the callose levels induced by the treatment. Instead, GrIP most likely functions by further elevating the accumulated amount of cdiGRP, the expression of which is up-regulated by the cadmium ions. Interestingly, the levels of cdiGRP mRNA were not affected by constitutive expression of GrIP, demonstrating that the enhancement in cdiGRP protein accumulation by GrIP overexpression occurs posttranslationally. Collectively, these observations suggest that GrIP interacts with cdiGRP and increases its level of accumulation; in turn, the elevated amounts of cdiGRP induce callose deposits in the plant cell walls. Therefore, GrIP and cdiGRP represent sequentially acting factors in a biochemical pathway that regulates callose accumulation in the plant vasculature.

Documentos Relacionados

• Sequence of cDNA for a novel light-induced glycine-rich protein.
• Expression of a gene encoding a glycine-rich protein in petunia.
• Developmental expression and localization of petunia glycine-rich protein 1.
• Specific localization of a plant cell wall glycine-rich protein in protoxylem cells of the vascular system
• Differential expression of five Arabidopsis genes encoding glycine-rich proteins.