Identification of an Operon Required for Ferrichrome Iron Utilization in Vibrio cholerae
AUTOR(ES)
Rogers, Marc B.
FONTE
American Society for Microbiology
RESUMO
Mutagenesis of Vibrio cholerae with TnphoA, followed by screening for fusions that were activated under low-iron conditions, led to the identification of seven independent fusion strains, each of which was deficient in the ability to utilize ferrichrome as a sole iron source for growth in a plate bioassay and had an insertion in genes encoding products homologous to Escherichia coli FhuA or FhuD. Expression of the gene fusions was independent of IrgB but regulated by Fur. We report here a map of the operon and the predicted amino acid sequence of FhuA, based on the nucleotide sequence. Unlike those of the E. coli fhu operon, the V. cholerae ferrichrome utilization genes are located adjacent and opposite in orientation to a gene encoding an ATP-binding cassette transporter homolog, but this gene, if disrupted, does not affect the utilization of ferrichrome in vitro.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=111292Documentos Relacionados
- Identification, cloning, and sequencing of a gene required for ferric vibriobactin utilization by Vibrio cholerae.
- HutZ Is Required for Efficient Heme Utilization in Vibrio cholerae
- Iron-vibriobactin transport system is not required for virulence of Vibrio cholerae.
- Identification of an iron-regulated virulence determinant in Vibrio cholerae, using TnphoA mutagenesis.
- Identification of additional genes required for O-antigen biosynthesis in Vibrio cholerae O1.