Identification of antigens recognized by T cells in human leishmaniasis: analysis of T-cell clones by immunoblotting.
AUTOR(ES)
Melby, P C
RESUMO
Immunity in human leishmaniasis is mediated by sensitized T lymphocytes; however, the antigens involved in eliciting this immunity have not been defined. We describe the generation of human T-lymphocyte clones derived from two patients with healed leishmaniasis. By use of one- and two-dimensional cellular immunoblotting techniques, we directly identified the parasite antigens recognized by these clones. To our knowledge, these are the first leishmanial antigens identified to which CD4+, gamma interferon-producing T cells from immune individuals have been shown to respond, the strategy may be of general use for the identification of antigens involved in immunity in this disease.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=260757Documentos Relacionados
- Profile of human T cell response to leishmanial antigens. Analysis by immunoblotting.
- Identification of T-cell epitopes on E2 protein of rubella virus, as recognized by human T-cell lines and clones.
- T-cell activation by autologous human T-cell leukemia virus type I-infected T-cell clones.
- Identification of two T-cell epitopes on the candidate Epstein-Barr virus vaccine glycoprotein gp340 recognized by CD4+ T-cell clones.
- Dengue virus-specific, human CD4+ CD8- cytotoxic T-cell clones: multiple patterns of virus cross-reactivity recognized by NS3-specific T-cell clones.