IDENTIFICATION OF COMPONENTS OF (NA+ + K+)-ADENOSINE TRIPHOSPHATASE BY DOUBLE ISOTOPIC LABELING AND ELECTROPHORESIS*

AUTOR(ES)

Chignell, Colin F.

RESUMO

A microsomal adenosine triphosphatase (ATPase) that requires both sodium and potassium ions is thought to be identical with, or an integral part of, the active cation transport system located in cell membranes. Attempts to isolate and purify (Na+ + K+)-ATPase have met with limited success because solubilization of microsomal protein causes partial, if not complete, loss of enzymatic activity. We now report the isolation from rat kidney microsomes of proteins which, though enzymatically inactive, could still be identified as components of the (Na+ + K+)-ATPase system.

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