Identification of Discrete Polyadenylate-containing RNA Components Transcribed from HeLa Cell Mitochondrial DNA*

AUTOR(ES)

Ojala, Deanna

RESUMO

Polyacrylamide gel electrophoresis and sedimentation analysis under denaturing conditions of poly(A)-containing RNA from the polysome region of the sedimentation pattern of a HeLa-cell mitochondrial lysate has revealed the occurrence of a discrete RNA component, which sediments in the native state with a sedimentation constant of about 7 S. From the sedimentation behavior under native and denaturing conditions and the poly(A) content, a molecular weight of about 9 × 104 has been estimated for this component. RNA·DNA hybridization experiments have indicated that this component is coded for by the light strand of mitochondrial DNA. Evidence for the occurrence of a poly(A)-containing RNA component sedimenting at about 9 S and coded for by the heavy strand has also been obtained.

Documentos Relacionados

• Turnover of polyadenylate-containing ribonucleic acid in Saccharomyces cerevisiae.
• Rate of Synthesis of Polyadenylate-Containing Ribonucleic Acid During the Yeast Cell Cycle
• Failure to detect "cap" structures in mitochondrial DNA-coded poly(A)-containing RNA from HeLa cells.
• In Vitro Translation of Polyadenylate-Containing RNAs from Dormant and Germinating Spores of the Fungus Botryodiplodia theobromae
• Detection of high levels of polyadenylate-containing RNA in bacteria by the use of a single-step RNA isolation procedure.