Identification of Novel Porcine Endogenous Betaretrovirus Sequences in Miniature Swine
AUTOR(ES)
Ericsson, Thomas
FONTE
American Society for Microbiology
RESUMO
PCR amplification of genomic DNA from miniature swine peripheral blood lymphocytes, using primers corresponding to highly conserved regions of the polymerase (pol) gene, allowed the identification of two novel porcine endogenous retrovirus (PERV) sequences, PMSN-1 and PMSN-4. Phylogenetic analyses of the nucleotide sequences of PMSN-1 and PMSN-4 revealed them to be most closely related to betaretroviruses. The identification of PERVs belonging to the Betaretrovirus genus shows that endogenous retroviruses of this family are more broadly represented in mammalian species than previously appreciated. Both sequences contained inactivating mutations, implying that these particular loci are defective. However, Southern blot analysis showed additional copies of closely related proviruses in the miniature swine genome. Analyses of fetal and adult miniature swine tissues revealed a broad mRNA expression pattern of both PMSN-1 and PMSN-4. The most abundant expression was detected in whole bone marrow c-kit+ (CD117+) progenitor bone marrow cells, fetal liver, salivary gland, and thymus. It appears unlikely that functional loci encoding these novel PERV sequences exist, but this remains to be established. The betaretrovirus sequences described in this report will allow such investigations to be actively pursued.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=115900Documentos Relacionados
- Identification of Exogenous Forms of Human-Tropic Porcine Endogenous Retrovirus in Miniature Swine
- Genotyping of Porcine Endogenous Retroviruses from a Family of Miniature Swine
- Porcine Endogenous Retrovirus Transmission Characteristics of an Inbred Herd of Miniature Swine
- Absence of Replication-Competent Human-Tropic Porcine Endogenous Retroviruses in the Germ Line DNA of Inbred Miniature Swine
- Identification of a Full-Length cDNA for an Endogenous Retrovirus of Miniature Swine