Identification of Salmonella somatic and flagellar antigens by modified serological methods.

AUTOR(ES)

Gruenewald, R

RESUMO

This report describes two modified methods for the identification of Salmonella somatic (O) and flagellar (H) antigens. Over a period of 2 years, both modified methods were found to be approximately three times less labor intensive than the standard methods while requiring no more technical skill. The modified methods were as accurate as the standard methods in identifying the O and H antigens of 350 Salmonella isolates. Furthermore, 43 O antisera reacted exclusively with organisms possessing homologous O antigens when the modified and two standard methods were used. At the antiserum dilutions used for H antigen identification, H antisera did not react with O antigens or heterologous H antigens by either the modified or the standard method. Compared with the standard method for H antigen identification, the modified method was approximately 20 times more economical with respect to antisera and usually generated a 1.5- to 4-fold higher titer. Since the antisera stored for use in the modified method for H antigen identification were usually 100-fold more dilute than the antisera stored for the standard method, an antibody-stabilizing buffer was incorporated in the diluted antisera, allowing these reagents to be used for at least 9 to 16 months.

Documentos Relacionados

• Somatic and Flagellar Immunofluorescence of Salmonella1
• Detection of modified nucleosides by rapid RNA sequencing methods.
• Demonstration of persistence of Salmonella typhimurium in an AIDS patient by molecular methods.
• Identification of Salmonella O antigens by coagglutination.
• SALMONELLA RUBISLAW WITH THREE “NORMAL” FLAGELLAR ANTIGENS