Identification of the defective genes in three mutant groups of influenza virus.

AUTOR(ES)

Ritchey, M B

RESUMO

Seven complementation-recombination groups of temperature-sensitive (ts) influenza WSN virus mutants have been previously isolated. Recently two of these groups (IV and VI) were shown to possess defects in the neuraminidase and the hemagglutinin gene, respectively, and two groups (I and III) were reported to have defects in the P3 and P1 proteins which are required for complementary RNA synthesis. In this communication we report on the defects in the remaining three mutant groups. Wild-type (ts+) recombinants derived from ts mutants and different non-ts influenza viruses were analyzed on RNA polyacrylamide gels. This technique permitted the identification of the P2 protein, the nucleoprotein, and the M protein as the defective gene products in mutant groups II, V, and VII, respectively. Based on the physiological behavior of mutants in groups II and V, it appears that P2 protein and nucleoprotein are required for virion RNA synthesis during influenza virus replication.

Documentos Relacionados

• Homologous interference mediated by defective interfering influenza virus derived from a temperature-sensitive mutant of influenza virus.
• Identification of hemagglutinin and neuraminidase genes of influenza B virus.
• Transcriptase activity and genome composition of defective influenza virus.
• Identification of the cap binding protein of influenza virus.
• Defective interfering virus associated with A/Chicken/Pennsylvania/83 influenza virus.