Identification of the in vitro translation products of adenovirus mRNA by immunoprecipitation.
AUTOR(ES)
Oberg, B
RESUMO
Adenovirus type 2 mRNA was translated in S30 extracts from Ehrlich ascites and wheat embryo cells. The in vitro products were identified by sodium dodecyl sulfate-gel electrophoresis after immunoprecipitation with specific antisera in the presence of urea. Seven virion polypeptides could be identified by immunoprecipitation. Three of these appear to be precursors to polypeptides of the virion. mRNA isolated late in adenovirus infection was separated into three size classes by zonal sedimentation. Material sedimenting at 26S was translated into polypeptides corresponding to the largest virion polypeptides II to IV, a 22S fraction corresponding to polypeptide V, and smaller polypeptides and a 15S fraction corresponding to polypeptide IX. A significant amount of polypeptide IX was also synthesized by the 26S and 22S RNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=354435Documentos Relacionados
- Respiratory syncytial virus proteins: identification by immunoprecipitation.
- Translation and identification of the mRNA species synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus.
- In vitro translation of adenovirus type 12-specific mRNA isolated from infected and transformed cells.
- Regulation of in vitro translation by double-stranded RNA in mammalian cell mRNA preparations.
- Adenovirus VA RNAI: a positive regulator of mRNA translation.
