Identification of the origin of replication of bovine papillomavirus and characterization of the viral origin recognition factor E1.
AUTOR(ES)
Ustav, M
RESUMO
Expression of the viral polypeptides E1 and E2 is necessary and sufficient for replication of BPV in mouse C127 cells. By providing these factors from heterologous expression vectors we have identified a minimal origin fragment from BPV that contains all the sequences required in cis for replication of BPV in short term replication assays. This same sequence is also required for stable replication in the context of the entire viral genome. The identified region is highly conserved between different papillomaviruses, and is unrelated to the previously identified plasmid maintenance sequences. The minimal ori sequence contains a binding site for the viral polypeptide E1, which we identify as a sequence specific DNA binding protein, but surprisingly, an intact binding site for the viral transactivator E2 at the ori is not required. The isolated origin shows an extended host region for replication and replicates efficiently in both rodent and primate cell lines.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=453185Documentos Relacionados
- Induction of structural changes in the bovine papillomavirus type 1 origin of replication by the viral E1 and E2 proteins.
- E1 recognition sequences in the bovine papillomavirus type 1 origin of DNA replication: interaction between half sites of the inverted repeats.
- Identification of a Short, Hydrophilic Amino Acid Sequence Critical for Origin Recognition by the Bovine Papillomavirus E1 Protein
- Binding of the E1 and E2 proteins to the origin of replication of bovine papillomavirus.
- The nucleotide sequence surrounding the origin of DNA replication of Col E1.