Immunization with Genetic Toxoids of the Arcanobacterium pyogenes Cholesterol-Dependent Cytolysin, Pyolysin, Protects Mice against Infection

AUTOR(ES)

Jost, B. Helen

FONTE

American Society for Microbiology

RESUMO

Pyolysin (PLO), a cholesterol-dependent cytolysin expressed by Arcanobacterium pyogenes, is an important host-protective antigen. However, this molecule is toxic and requires inactivation prior to its use as a vaccine. Three genetically toxoided, nonhemolytic PLO molecules, HIS-PLO.F497, HIS-PLO.ΔP499, and HIS-PLO.A522, were found to be nontoxic, and vaccinated mice were protected from infection, indicating the potential of these toxoids as vaccines. Furthermore, in a mouse model of infection, A. pyogenes carrying the F497 mutation was as attenuated as a PLO-deficient strain, indicating that the cytolytic activity of PLO is important in virulence.

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