Immunization with major outer membrane protein (porin) preparations in experimental murine salmonellosis: effect of lipopolysaccharide.

AUTOR(ES)

Kuusi, N

RESUMO

A crude major outer membrane (porin) preparation, obtained from a rough strain of Salmonella typhimurium earlier shown to be protective both in active and passive immunization of mice against challenge with smooth S. typhimurium, was further purified. Removal of the main impurities, lipopolysaccharide (LPS) and lipoprotein, was accompanied by loss of protective capacity in passive immunization experiments. Reconstitution with rough LPS restored the protective capacity. Protection was, however, concluded not to be due to anti-LPS, because a large fraction of the anti-LPS antibodies could be removed from the protective rabbit antiserum with an LPS immunosorbent without loss of protection.

Documentos Relacionados

• An outer membrane protein (porin) as an eliciting antigen for delayed-type hypersensitivity in murine salmonellosis.
• Immunization with major outer membrane proteins in experimental salmonellosis of mice.
• Experimental Salmonellosis: Hypersensitivity to Cell Wall Lipopolysaccharide and Anti-Infectious Resistance of Mice Infected with Salmonella
• Pathogenesis of experimental salmonellosis: inhibition of protein synthesis by cytotoxin.
• Use of a purified outer membrane protein F (porin) preparation of Pseudomonas aeruginosa as a protective vaccine in mice.