Immuno-electron microscopic localization of lipopolysaccharide antigens on ultrathin sections of Salmonella typhimurium.

AUTOR(ES)

Takamiya, H

RESUMO

Lipopolysaccharide antigens were demonstrated on ultrathin sections of styrene-embedded Salmonella typhimurium by direct postembedding staining with ferritin-labeled antibodies. The antigenicity, partially masked in the embedding process, could be satisfactorily recovered by treatment of ultrathin sections with nonspecific protease. As judged from the reaction site of the ferritin-labeled antibodies, the lipopolysaccharides were localized in two zones. The broader zone of densely distributed ferritin molecules was superimposed over the whole outer cellwall, and a smaller zone revealing antigenicity was found over the cell membrane, which strongly supports the concept that the latter is the site of synthesis of lipopolysaccharides. The well-defined labeled areas between these two antigenic zones may be the routes whereby the synthesized polysaccharide molecules reach the cell wall.

Documentos Relacionados

• Immuno-electron microscopic study on the antigenicity of tubular structures associated with human rotavirus.
• Immuno-Electron Microscopy of the Morphogenesis of Mumps Virus
• Localization of Tamm-Horsfall glycoprotein in the human kidney using immuno-fluorescence and immuno-electron microscopical techniques.
• Electron microscopic visualization of trp operon expression in Salmonella typhimurium.
• Visualization of Z sequences in form V of pBR322 by immuno-electron microscopy.