Immunochemical detection of a common antigen among Streptococcus uberis isolates.

AUTOR(ES)

Jones, K F

RESUMO

Fifty-three isolates of Streptococcus uberis from various sources were examined for the presence of a common antigen. Initially, a serum was produced in rabbits which, by using rocket line immunoelectrophoresis, proved to react with identity to all of the S. uberis crude extracts as well as group B and E streptococcal extracts. The antigen(s) responsible for this cross-reactivity was partially purified by Sephacryl S-200 gel chromatography and analyzed by fused rocket immunoelectrophoresis. Further analysis by immunodiffusion showed that probably two antigens in the gel chromatography-consolidated fractions were common to the S. uberis and group B and E isolates, but that one of the antigens present was unique to S. uberis. Trypsin destroyed the immunoreactivity of this antigen. Isolation of this common antigen could possibly alleviate some of the tedium associated with the identification of this organism.

Documentos Relacionados

• Major surface antigen p190 of Plasmodium falciparum: detection of common epitopes present in a variety of plasmodia isolates.
• Identification of a Streptococcus agalactiae protein antigen associated with bovine mastitis isolates.
• The HpmA hemolysin is more common than HlyA among Proteus isolates.
• Diversity among Helicobacter pylori isolates.
• Molecular Epidemiology of Streptococcus uberis Isolates from Dairy Cows with Mastitis