Immunoglobulin enhancer and promoter motifs 5' of the B29 B-cell-specific gene.

AUTOR(ES)

Hermanson, G G

RESUMO

B29 is a B-cell-specific member of the immunoglobulin gene superfamily that is expressed throughout B-cell development beginning with the earliest precursor B cells undergoing immunoglobulin heavy-chain gene segment rearrangements. We have analyzed the region upstream of the B29 gene to identify DNA sequences involved in transcriptional regulation of this gene. The B29 gene lacks a TATA box and transcription is initiated at multiple sites. The B29 gene sequence 5' of these transcription start sites contains six promoter and enhancer motifs known to control immunoglobulin gene transcription. The most notable is a perfect octamer (5'-ATTTGCAT-3'), which binds the Oct-2 B-cell-specific transcription factor and thereby can account for the tissue-specific expression of this gene.

Documentos Relacionados

• Multiple motifs regulate the B-cell-specific promoter of the B29 gene.
• Characterization of the human immunoglobulin kappa gene 3' enhancer: functional importance of three motifs that demonstrate B-cell-specific in vivo footprints.
• Positive and negative regulation of immunoglobulin gene expression by a novel B-cell-specific enhancer element.
• Anatomy of a new B-cell-specific enhancer.
• Two conserved essential motifs of the murine immunoglobulin lambda enhancers bind B-cell-specific factors.