Immunoglobulin Synthesis and Secretion, IV. Sites of Incorporation of Sugars as Determined by Subcellular Fractionation*
AUTOR(ES)
Uhr, Jonathan W.
RESUMO
P3K cells were uniformly labeled with [14C]leucine and also for 1 hr with [3H]leucine, [3]galactose, or [3H]glucosamine. Subcellular fractionation of labeled cells was performed and the amounts of radioactive immunoglobulin G in each fraction were determined. The results indicate that amino acids are incorporated in the rough endoplasmic reticulum, galactose primarily in the smooth endoplasmic reticulum, and glucosamine at both sites. A small portion of radioactivity was found in the microsomal supernatant regardless of the radioisotope employed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=283143Documentos Relacionados
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